KMID : 0616120230540010001
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Korean Journal of Pharmacognosy 2023 Volume.54 No. 1 p.1 ~ p.8
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Effects of Esculetin on TNF-¥á Induced MMP-1 Expression in Human Fibroblasts Hs68
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Jeon Bo-Hee
Kim Yong-Min
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Abstract
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The skin is an important barrier that protects the body from harmful environments such as UV rays. When the skin is repeatedly stimulated, such as UV rays, ROS and pro-inflammatory cytokines are overproduced. As a result, the proteins and nucleic acids that make up the skin are damaged, and aging occurs. Esculetin is known to have anti-inflammatory, antioxidant and UV-induced MMP-1 inhibitory effects. However, the inhibitory effect of MMP-1 on TNF-¥á-induced fibroblasts is not known. Therefore, in this study, the MMP-1 inhibitory effect of esculetin was confirmed in TNF-¥á-induced fibroblasts. As a result of confirming the cytotoxicity of esculetin in Hs68 cells by MTT assay, there was no significant toxicity up to 200 ¥ìM. As a result of real-time PCR and ELISA, it was confirmed that esculetin inhibited the expression of MMP-1. Esculetin did not inhibit MAPK (ERK, JNK, p38) phosphorylation, but inhibited phosphorylation of the mTOR-p70S6k signaling pathway. In addition, it was confirmed that the phosphorylation of the transcription factor NF-¥êB was inhibited. These results suggest that esculetin has potential as an anti-aging material.
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KEYWORD
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Esculetin, MMP-1, MAPK, mTOR-p70S6K, NF-¥êB
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